Each enzyme works better under optimum conditions, which favor the most active form for the enzyme molecule. Enzyme and substrate concentration, temperature, and pH are environmental factors of import by bring forthing the most reaction rate. Besides, the different of factors are used to analyze the effects of catalase and human amylase. At C and pH 7 of catalase, the concentration of substrates and enzyme addition and the rates of reaction besides rise.
The presence of an enzyme within a cell is essential in order for any sort of reaction to take place. In such a homeostatic environment, the temperature and the pH concentration of hydrogen ionsremain within a fairly narrow range.
Extreme variations in pH and temperature denature the enzyme by altering its chemical structure, thus adversely affecting the chemical reaction. The enzyme, catalase, reacts with hydrogen peroxide in order to yield water and oxygen. Therefore, upon reaction, bubbling will occur as oxygen is produced.
The amount of bubbling, or oxygen that is produced is dependent on the conditions under which the reaction takes place or fails to. Purpose The Purpose of this experiment was to determine the optimal temperature and pH levels under which enzymatic reactions involving the enzyme catalase and the reactant, hydrogen peroxide, may successfully occur.
Hypothesis It is hypothesized that because extreme variations in temperature and pH will negatively affect the reaction, mild temperatures and more neutral ph levels will yield the best results most bubbles.
Upon preparation for the tests, the three temperature test tubes were marked at the 1 cm and 5 cm levels, while the three pH test tubes were marked at the 1, 3, and 7 cm levels. To test for temperature, each tube was placed in a separate location with varying temperature: After placing each tube in its experimental setting, 15 minutes elapsed before removing them.
Immediately after removing the tubes, hydrogen peroxide was added to each tube up to the second 5 cm mark. They were then swirled to mix, and after fifteen seconds, the bubble columns in each vile were measured in millimeters, and then recorded in a data table.
The procedure for analyzing pH levels was rather different.
The three test tubes, marked at three different levels, were each filled to the first mark 1cm with catalase, and to the second mark 3 cm with water. However, the pH of the water had been adjusted differently for each vile.
The first tube received water adjusted to pH three, the second received water adjusted to pH seven, and third received pH eleven. Consequently, each tube was then filled to the last mark 7 cm with hydrogen peroxide, swirled to mix and then observed after twenty seconds.
After twenty seconds passed, the bubble columns of each of he tubes were measured in millimeters. Results were recorded on a chart. RESULTS As expected, during the temperature test, the least amount of bubbles were emitted in tubes that had been exposed to extreme heat.
The tube placed in boiling water yielded zero millimeters of bubbles. The boiling water denatured the enzyme and altered its chemical structure rendering it incapable of undergoing metabolic reactions.Influence Of Temperature On Activity Of Liver Catalase Biology Essay Biology Lan – Factors Affecting Enzyme Activity Investigation into the effect of different ph levels and hydrogen peroxide concentrations on the activity of potato tissue catalase.
Title: THE EFFECT OF PH ON THE ACTIVITY OF CATALASE Aim: To study the effect of pH on the activity of catalase. Introduction: Catalase, an enzyme found in many different tissues, catalyses the breakdown of hydrogen peroxide into water and oxygen.
The biocatalyst activity is influenced by a number of factors, such as affinity and substratum concentration, temperature, pH-medium, the presence of activators and inhibitors.
Experts identify four types of elements that determine the catalytic spree of an enzyme. The Effect of pH on the Activity of the Enzyme Amylase Essay Words | 5 Pages. The Effect of pH on the Activity of the Enzyme Amylase Aim: To find the effect of pH on the activity of the enzyme amylase.
An enzyme is a type of protein found in all living cells. Effects of Temperature and pH on Catalase Activity INTRODUCTION Enzymes are organic catalysts that spur metabolic reactions. The presence of an enzyme within a cell is essential in order for any sort of reaction to take place.5/5(1).
HYPOTHESISDifferent pH values influence the activity of catalase. It was obtained from the literature, that the range of pH values, at which catalase works is approximately 4 to 9, with an optimum pH of /5(2).